Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted

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Biphenyl dioxygenase from an arctic isolate is not cold adapted.

Biphenyl dioxygenase from the psychrotolerant bacterium Pseudomonas sp. strain Cam-1 (BPDO(Cam-1)) was purified and found to have an apparent k(cat) for biphenyl of 1.1 +/- 0.1 s(-1) (mean +/- standard deviation) at 4 degrees C. In contrast, BPDO(LB400) from the mesophile Burkholderia xenovorans LB400 had no detectable activity at this temperature. At 57 degrees C, the half-life of the BPDO(Cam...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 2008

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.02928-07